DEVELOPMEN OF A NEW DNA SEQUENCING INSTRUMENT

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 11261
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1989
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Sybtrel Biotechnology
3330 Causeway Blvd #433, Matairie, LA, 70002
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE OBJECTIVE OF THIS PROJECT IS TO DEVELOP A DNA SEQUENCING INSTRUMENT UTILIZING THE PYROPHOSPHATE-BASED METHOD OF DNA SEQUENCING. THIS IS A NEW METHOD THAT DOES NOT USE ELECTROPHORESIS, RADIOACTIVITY, OR FLUORESCENCE. THE METHOD WORKS BY MEASURING PYROPHOSPHATE GENERATED BY THE DNA POLYMERIZATION REACTION. THE PYROPHOSPHATE IS MEASURED CONTINUOUSLY BY A DEVICE CONSISTING OF A SERIES OF COLUMNS CONTAINING ENZYMES COVALENTLY ATTACHED TO A SOLIDSUPPORT. PHASE I WILL BE DEVOTED TO SOLVING THE REMAINING BIOCHEMICAL PROBLEMS ASSOCIATED WITH THE METHOD. IF THE METHOD IS DEMONSTRATED TO WORK WELL ENOUGH, PHASE II WILL CONSIST OF CONSTRUCTION OF A PROTOTYPE INSTRUMENT SUITABLE FOR LARGE-SCALE PRODUCTION.

* information listed above is at the time of submission.

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