DEVELOPMEN OF A NEW DNA SEQUENCING INSTRUMENT

Award Information
Agency:
Department of Health and Human Services
Amount:
$50,000.00
Program:
SBIR
Contract:
N/A
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
1989
Phase:
Phase I
Agency Tracking Number:
11261
Solicitation Topic Code:
N/A
Small Business Information
Sybtrel Biotechnology
3330 Causeway Blvd #433, Matairie, LA, 70002
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE OBJECTIVE OF THIS PROJECT IS TO DEVELOP A DNA SEQUENCING INSTRUMENT UTILIZING THE PYROPHOSPHATE-BASED METHOD OF DNA SEQUENCING. THIS IS A NEW METHOD THAT DOES NOT USE ELECTROPHORESIS, RADIOACTIVITY, OR FLUORESCENCE. THE METHOD WORKS BY MEASURING PYROPHOSPHATE GENERATED BY THE DNA POLYMERIZATION REACTION. THE PYROPHOSPHATE IS MEASURED CONTINUOUSLY BY A DEVICE CONSISTING OF A SERIES OF COLUMNS CONTAINING ENZYMES COVALENTLY ATTACHED TO A SOLIDSUPPORT. PHASE I WILL BE DEVOTED TO SOLVING THE REMAINING BIOCHEMICAL PROBLEMS ASSOCIATED WITH THE METHOD. IF THE METHOD IS DEMONSTRATED TO WORK WELL ENOUGH, PHASE II WILL CONSIST OF CONSTRUCTION OF A PROTOTYPE INSTRUMENT SUITABLE FOR LARGE-SCALE PRODUCTION.

* information listed above is at the time of submission.

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