A HUMAN IL-1 INHIBITOR FOR THE TREATMENT OF SEPTIC SHOCK

Award Information
Agency: Department of Defense
Branch: Navy
Contract: N/A
Agency Tracking Number: 9168
Amount: $49,326.00
Phase: Phase I
Program: SBIR
Awards Year: 1988
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
1885 - 33rd St, Boulder, CO, 80301
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 DR CHARLES H HANNUM
 (303) 938-6200
Business Contact
Phone: () -
Research Institution
N/A
Abstract
WE PROPOSE TO PURIFY AN IL-1 INHIBITOR FROM THE SUPERNATANT OF MONOCYTES CULTURED ON PLATES COATED WITH IGG. THIS INHIBITORY ACTIVITY HAS BEEN SHOWN TO BE ABOUT 22 KILODALTONS IN MOLECULAR WEIGHT AND TO INHIBIT IL-1 IN SEVERAL ASSAYS, NAMELY IL-1 MEDIATED THYMOCYTE PROLIFERATION, COLLAGENASE RELEASE BY ARTICULAR CHONDROCYTES, AND PGE(2) RELEASE BY BOTH SYNOVIAL AND DERMAL FIBROBLASTS. WE WILL PRODUCE LARGE QUANTITIES OF THE INHIBITOR-CONTAINING SUPERNATANT AND PURIFY THE PROTEIN BY CONVENTIONAL CHROMATOGRAPHIC TECHNIQUES. INITIALLY WE WILL USE THE THYMOCYTE-PROFILERATION ASSAY TO MONITOR PURIFICATION BUT WILL IMMEDIATELY BEING DEVELOPING A FASTER PGE(2)-RELEASE ASSAY TO SPEED UP PURIFICATION. WE ALSO WILL BEGIN DEVELOPING MONOCLONAL ANTIBODIES TO THIS MOLECULE. OUR PHASE-I GOALS ARE TO DEVELOP A RAPID ASSAY FOR THIS INHIBITOR AND TO ATTAIN A 400-FOLD PURIFICATION.

* Information listed above is at the time of submission. *

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