A HUMAN IL-1 INHIBITOR FOR THE TREATMENT OF SEPTIC SHOCK

Award Information
Agency:
Department of Defense
Branch
Navy
Amount:
$49,326.00
Award Year:
1988
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
9168
Agency Tracking Number:
9168
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1885 - 33rd St, Boulder, CO, 80301
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DR CHARLES H HANNUM
(303) 938-6200
Business Contact:
() -
Research Institute:
n/a
Abstract
WE PROPOSE TO PURIFY AN IL-1 INHIBITOR FROM THE SUPERNATANT OF MONOCYTES CULTURED ON PLATES COATED WITH IGG. THIS INHIBITORY ACTIVITY HAS BEEN SHOWN TO BE ABOUT 22 KILODALTONS IN MOLECULAR WEIGHT AND TO INHIBIT IL-1 IN SEVERAL ASSAYS, NAMELY IL-1 MEDIATED THYMOCYTE PROLIFERATION, COLLAGENASE RELEASE BY ARTICULAR CHONDROCYTES, AND PGE(2) RELEASE BY BOTH SYNOVIAL AND DERMAL FIBROBLASTS. WE WILL PRODUCE LARGE QUANTITIES OF THE INHIBITOR-CONTAINING SUPERNATANT AND PURIFY THE PROTEIN BY CONVENTIONAL CHROMATOGRAPHIC TECHNIQUES. INITIALLY WE WILL USE THE THYMOCYTE-PROFILERATION ASSAY TO MONITOR PURIFICATION BUT WILL IMMEDIATELY BEING DEVELOPING A FASTER PGE(2)-RELEASE ASSAY TO SPEED UP PURIFICATION. WE ALSO WILL BEGIN DEVELOPING MONOCLONAL ANTIBODIES TO THIS MOLECULE. OUR PHASE-I GOALS ARE TO DEVELOP A RAPID ASSAY FOR THIS INHIBITOR AND TO ATTAIN A 400-FOLD PURIFICATION.

* information listed above is at the time of submission.

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