Centrifugal Cell-Retention Bioreactor
Small Business Information
P. O. Box 5056, Hanover, NH, 03755
Robert C. Dean Jr.
AbstractIn the production of large, complex therapeutic proteins, animal cells are preferred as the biocatalyst. Various types of production bioreactors are employed including deep-stirred and sparged tanks (with or without microcarriers), cell-immobilizing matrices, and fluidized beds of porous microcarriers. We have invented a new cell-retention technique using a centrifuge to remove and return living cells from the harvest to the bioreactor. According to the literature, this is a first-time achievement. Preliminary tests indicate high cell density, viability, cell-specific productivity relative to other animal-cell bioreactors. The objective of Phase I is to test this invention beyond the single cell line that has been tested in the laboratory to establish its feasibility as an economically important bioreactor for large-scale animal-cell culture in the production of therapeutic proteins. An extension of the work in Phase II will confirm its economic value at beta-sites in the biopharmaceutical industry and will extend its application to plant and insect cells. In Phase III, the centrifugal cell-retention bioreactor will be commercialized to become an important new bioprocessing tool.
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