Snap-To-It Probes

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$1,164,115.00
Award Year:
2007
Program:
SBIR
Phase:
Phase II
Contract:
2R44CA094612-03
Award Id:
59963
Agency Tracking Number:
CA094612
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
SYNTRIX BIOSYSTEMS, INC., 215 CLAY ST NW, STE B-5, AUBURN, WA, 98001
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
114845659
Principal Investigator:
JOHN ZEBALA
(253) 833-8009
JZEBALA@SYNTRIXBIO.COM
Business Contact:
CHRIS MA
() -
dhoeke@syntrixbio.com
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): Microarrays offer the potential to profile genetic alterations in a highly multiplexed format, but have suffered from sub-optimal specificity of target capture. Array technologies which improve the specificity of target capture will be of commercial value by allowing the manufacture of arrays with superior performance characteristics. We propose to develop a novel Snap-To-It array technology that consists of arrayed PNA and DNA probes that are conformationally constrai ned by an intra-molecular chelate. Binding to target results in the chelate dissociation and the probe snapping to the target nucleic acid in what is an all-or-none mechanism. Consistent with previous, solution phase, thermodynamic studies using Snap-To -It probes, we hypothesize that constrained probes immobilized on an array will exhibit superior target specificity compared to unconstrained probes. The proposed chelate motifs are easily introduced into arrays using existing standard array synthesis met hods with only a few additional process steps. Further, chelate binding is orthogonal to Watson-Crick pairing, and thus will not interfere with target hybridization. Phase I demonstrated feasibility of Snap-To-It probes and set the stage to move into an intensive Phase II program involving the development and testing of prototype Snap-To-It arrays for cancer analysis. DNA probes in solution and in high-throughput microarrays are invaluable tools used in areas from clinical diagnostics to fundament al research. The Snap-To-It probe technology we propose will produce microarrays with superior performance characteristics.

* information listed above is at the time of submission.

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