PRODUCTION OF RECOMBINANT HTLV-II ENVELOPE PROTEIN IN E. COLI

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 13744
Amount: $45,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
950 Boston Ave, Longmont, CO, 80501
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Yitzhak Stabinsky
 (303) 651-6419
Business Contact
Phone: () -
Research Institution
N/A
Abstract
HUMAN T-CELL LEUKEMIA VIRUS TYPE II (HTLV-II) IS CONSIDERED TO BE AN ETIOLOGIC AGENT OF HAIRY CELL LEUKEMIA, AND ITS PREVALENCE IS INCREASING IN THE BLOOD SUPPLY. THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO PRODUCE AN IMMUNOLOGICAL ASSAY TO STUDY ITS PREVALENCE IN THE BLOOD SUPPLY. THIS PHASE I RESEARCH SPECIFICALLY AIMS TO DEVELOP A RECOMBINANT DNA SYSTEM TO PRODUCE A PORTION OF THE ENVELOPE PROTEIN THAT IS IMMUNOGENIC. THE APPROACH TO BE USED WILL INVOLVE THE ASSEMBLY OF A SYNTHETIC GENE CODING FOR THAT PORTION OF THE ENVELOPE PROTEIN. THE GENE WILL BE CLONED AND EXPRESSED IN E. COLI. HIGH-LEVEL EXPRESSION OF THE DESIRED PRODUCT WILL ENABLE PHASE II RESEARCH TO FOCUS ON THE E. COLI FERMENTATION SCALE-UP AND PURIFICATION.

* Information listed above is at the time of submission. *

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