Autosomal Sex Reversal of Rainbow Trout

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: 2007-33610-17936
Agency Tracking Number: 2007-00412
Amount: $76,300.00
Phase: Phase I
Program: SBIR
Awards Year: 2007
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
PO Box 1290, Sumner, WA, 98390
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 James Parsons
 (253) 863-9463
 parsons@troutlodge.com
Business Contact
 Jim Barfoot
Title: President
Phone: (253) 863-0446
Email: jbarfoot1@aol.com
Research Institution
N/A
Abstract
The proposed SBIR project seeks to develop an alternative approach to the use of hormone treatment (17 -methyltestosterone) of broodstock for the production of all-female rainbow trout. In the course of normal spawning of large numbers of rainbow trout, Troutlodge personnel have identified rare occurrences of males in populations of XX female stocks. Previous studies using common carp in the Netherlands and rainbow trout in France have identified similar rare male individuals and determined that they are the result of homozygosity for a recessive autosomal sex-determining allele that overrides the normal XX-XY sex determination mechanism. This project will test for a similar phenomenon in Troutlodge stocks of rainbow trout, and utilize these alleles to establish male lines of XX individuals without the use of testosterone analogs. Rare males will be tested for (1) their ability to produce all female progeny in crosses with normal females, and (2) their ability to produce significant numbers of male progeny when their sperm is used for androgenesis (all-paternal inheritance). If both objectives are successful, it will indicate that autosomal sex reversal is likely and lead directly to Phase II research.

* Information listed above is at the time of submission. *

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