Development of a Fluorometric In Vitro Assay for HIV

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$98,224.00
Award Year:
1995
Program:
SBIR
Phase:
Phase I
Contract:
1 R43 AI37463-01,
Agency Tracking Number:
29112
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Trevigen, Inc.
8405 Helgerman Court, Gaithersburg, MD, 20877
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Philip Vanek
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
The function of retroviral integrase (IN) is the integration of viral DNA into host cell DNA, an esretroviral replication. IN protein recognizes viral cognate at sites, removes two nucleotides from tviral DNA (3' processing) and joins the 3'-processed cognate at sites to the newly created 5' ends otransfer). This research will develop a fluorometric assay utilizing fluorescence resonance energy tprimarily the result of dipole-dipole interactions between the donor and the acceptor. Hence, when afluorophores are introduced to a DNA substrate in close proximity, the donor fluorescence will be quThe process will be fully reversed if fluorophores are physically separated by enzyme-mediated DNA cquenched fluorescence will be recovered and the extent of this change upon cleavage of fluorescent Dreflect the extent of the cleavage reaction of DNA. This assay is non radioactive, sensitive, rapid,possible to obtain precise kinetic information essential for mechanistic studies and screening of en

* information listed above is at the time of submission.

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