Lateral Flow Assay for Oncogenic Strains of HPV

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$107,000.00
Award Year:
2003
Program:
SBIR
Phase:
Phase I
Contract:
1R43CA103151-01
Award Id:
65412
Agency Tracking Number:
CA103151
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
TREVIGEN, INC., 8405 HELGERMAN CT, GAITHERSBURG, MD, 20877
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
LEONARDBAZAR
(301) 216-2800
LBAZAR@TREVIGEN.COM
Business Contact:
MICHAELELLIOTT
(301) 216-2800
MELLIOTT@TREVIGEN.COM
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): Trevigen/TreviMed has developed the Mismatch Identification DNA Analysis System (MIDAS) that relies on SNIPase (genetically-optimized DNA mismatch repair enzymes) for the detection of pathogen DNA without the need for PCR amplification. The purpose of the research outlined in this Phase I proposal is to adapt MIDAS to a lateral-flow dipstick platform. To this end, we propose the following specific aims: (1) to design and test immobilized MIDAS probes to high-risk oncogenic strains of human papilloma virus (HPV strains 16 and 18) that incorporate biotin and various haptens such as dinitrophenyl (DNP), digoxigenin (DIG), fluorescein isothiocyanate (FITC), or bromodeoxyuridine (BrdU). In the presence of HPV target DNA and SNIPase, cleavage of the probe occurs, leading to the formation of a colored band on a lateral-flow dipstick. The MIDAS probes will be optimized initially with single-stranded oligonucleotides as targets, corresponding to HPV-16 and HPV-18 DNA sequences; (2) to optimize Dipstick-MIDAS for detecting the presence of cloned HPV-16 and HPV-18 DNA, and genomic DNA derived from HPV-16 and HPV-18-positive cell lines. These efforts will culminate in a rapid, sensitive, multiplexed, cost-effective, and accurate assay for oncogenic strains of HPV that cause cervical cancer.

* information listed above is at the time of submission.

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