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Chemically Modified dNTPs as a General Approach for Improved Hot Start PCR

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43GM079836-01
Agency Tracking Number: GM079836
Amount: $99,889.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: 2007
Award Year: 2007
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
United States
DUNS: 945720043
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (858) 546-0004
Business Contact
Phone: (858) 546-0004
Research Institution

DESCRIPTION (provided by applicant): The polymerase chain reaction (PCR) is a powerful technique used to amplify a DNA sequence of interest. Continual advancements to the technique have included development of reverse-transcriptase PCR and real- time PCR technologies. With the advent of these and other technologies that allow for accurate quantitation of a sequence of interest, there are continual needs for improvements to the accuracy of the technique. Herein we propose the development of a novel Hot Start PCR strategy which may improve the specificity in PCR by reducing the number of undesired amplification products. Although numerous Hot Start PCR technologies have been developed, none of these utilize chemically-modified synthetic deoxynucleoside triphosphates (dNTPs). The present proposal aims to further explore the feasibility of using modified dNTPs that inhibit primer extension until a "Hot Start" preheating step is performed. It is anticipated that this approach to Hot Start PCR should be amenable to existing PCR technologies. This may allow application for use with existing PCR systems, by simple substitution of the unmodified dNTP mix with the corresponding modified dNTP mix. Overall, we propose the development of a novel approach to Hot Start PCR that will offer an added level of specificity to nucleic acid amplification. The polymerase chain reaction (PCR) is a molecular biology technique that is becoming routinely used in the field of molecular diagnostics. In this field, accurate identification of DNA sequences is imperative for reliable diagnosis of disease. To further improve the accuracy of the PCR technique, we propose the investigation of a novel Hot Start PCR approach, in which the dNTP substrates are chemically modified.

* Information listed above is at the time of submission. *

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