Real Time Monitoring of Signaling Pathways in Biological Cells

Award Information
Agency: Department of Defense
Branch: Defense Advanced Research Projects Agency
Contract: W31P4Q-05-C-0227
Agency Tracking Number: 04SB1-0236
Amount: $799,968.00
Phase: Phase II
Program: SBIR
Awards Year: 2005
Solicitation Year: 2004
Solicitation Topic Code: SB041-013
Solicitation Number: 2004.1
Small Business Information
4401 Dayton-Xenia Road, Dayton, OH, 45432
DUNS: 074689217
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Melanie Tomczak
 Research Scientist
 (937) 426-6900
 mtomczak@ues.com
Business Contact
 Francis Williams, Jr.
Title: Contracts Manager
Phone: (937) 426-6900
Email: fwilliams@ues.com
Research Institution
N/A
Abstract
Cell function and survival are dictated by the transduction of the appropriate signaling pathways within the cell. These signaling pathways regulate nearly every aspect of cellular function from growth and proliferation, to progammed cell death, and sensing the surrounding environment. Until recently, investigation of cellular pathways has relied on destructive or after-the-fact techniques, including probing cell lysate for changes after a particular agonist has been added to the cell culture, or inferring protein activity in a pathway based on downstream events. We propose to investigate, in real time and in vivo, cellular pathways with a nanoprobe that can release or capture an enzyme or protein on demand. This probe is based on TiO2 nanoparticles, which are photoexcitable in the UV-range, and are attached to a molecular wire, through which this excitation can be harnessed. With modifications to this nanoprobe, it should be possible also to regulate gene transcription and use the nanoprobe in a biosensor platform to monitor the environment or solutions (including biologically-based solutions) for chemical or biological weapons.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government