Real Time Monitoring of Signaling Pathways in Biological Cells

Award Information
Agency: Department of Defense
Branch: Defense Advanced Research Projects Agency
Contract: W31P4Q-04-C-R207
Agency Tracking Number: 04SB1-0236
Amount: $98,917.00
Phase: Phase I
Program: SBIR
Awards Year: 2004
Solicitation Year: 2004
Solicitation Topic Code: SB041-013
Solicitation Number: 2004.1
Small Business Information
4401 Dayton-Xenia Road, Dayton, OH, 45432
DUNS: 074689217
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Melanie Tomczak
 Research Scientist
 (937) 426-6900
Business Contact
 Francis Williams
Title: Contracts Manager
Phone: (937) 426-6900
Research Institution
Signaling pathways in cells historically have been studied using in vitro assays, where components are purified from cells and the reactions are done in a test tube. These assays can be helpful in identifying key players of signaling pathways, however these in vitro assays cannot mimic the complex environment and interactions found in the cytoplasm of an intact cell. Several important signaling pathways involved in maintaining healthy tissue and preventing disease are the kinases, which phosphorylate proteins in response to specific upstream signals. Recent advances in nanoparticle technology are starting to make visualization of cellular events in vivo, in real time, possible. We propose to use two different bioconjugated nanoparticles (BNPs) to visualize and localize kinase activity, using one BNP, and, with the second BNP, we will specifically inhibit kinase activity and release that inhibition with a specific, external signal.

* Information listed above is at the time of submission. *

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