Rapid Detection of Aflatoxin B1 with Reusable Immunochemical Optrodes

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: N/A
Agency Tracking Number: 30528
Amount: $54,939.00
Phase: Phase I
Program: SBIR
Awards Year: 1995
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
5258 Veterans Blvd., Suite D, Metairie, LA, 70006
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
 Mr. Robert M. Carter
Phone: () -
Research Institution
Fiber optic measurement of aflatoxins will be performed suing optrodes specific for aflatoxin. One optrode will consist of aflatoxin linked by adsorption or immobilized by sulfhydryl groups on the tip of a fiber optic bundle. For determination of aflatoxin using immobilized F(ab') antibody, the antibody will first be cleaved and then immobilized to quartz plates or wafers by silinization. Aflatoxin-antibody conjugates, in which aflatoxin has a native fluorescence occurring at 362 nm, will be measured directly against a standard curve using a fluorimeter. Another scheme, in which the aflatoxin antibody is immobilized and then labeled with FITC or chemiluminescent tag (isoluminol), will be quenched due to the presence of free aflatoxin. Alternatively, an aflatoxin-oxime can be labeled with either FITC, through a isothiocyanate linkage, or with isoluminol glutaraldehyde condensation and immobilized on the fiber optic bundle. Aflatoxin antibody will be co-incubated with known (spiked) or unknown samples before measurement. As free aflatoxin concentrations increase, signals increase in a proportional manner. These measurements will be conducted with a Lumac Biocounter and plotted against a known standard curve. Optrode accuracy will be determined against standard in-house ELISA and HPLC methods performed at local USDA or FDA laboratories.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government