Tissue Dissociation Enzymes for Islets and Other Cells

Award Information
Department of Health and Human Services
Award Year:
Phase II
Award Id:
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
1102 Indiana Avenue, INDIANAPOLIS, IN, 46202
Hubzone Owned:
Minority Owned:
Woman Owned:
Principal Investigator:
(317) 269-7142
Business Contact:
() -
Research Institute:
DESCRIPTION (provided by applicant): VitaCyte LLC is working to improve tissue dissociation enzymes (TDE) required for the isolation of insulin producing pancreatic islet cells from both human and porcine organs. Transplantation of islets into Type 1 Diabe tic patients represents a promising therapy as means to replace administration of insulin by external factors. The development of the Edmonton Protocol demonstrated insulin independence in patients transfused with purified pancreatic islets. Insufficient i slet yields arising from inconsistencies in TDE manufacturing have plagued continuing attempts to transfer this promising therapy to routine clinical practice. Improved biochemical assays have been used to correlate collagenase molecular forms with tissue dissociation. These data indicate the importance of each molecular form for different tissue types. The goal of this project is to develop a robust and reproducible manufacturing process using recombinant TDE that will improve the quality and commercial av ailability for both human allotransplantation and porcine xenotransplantation. Specifically, three recombinant constructs of Clostridia histolyticum collagenases: intact Class 2 (C2intact), intact Class 1 with two collagen binding domains (C1intact), and d egraded C1 with a single collagen binding domain (C1100kDa) will be generated using standard molecular biology techniques. A large scale bacterial fermentation and expression production protocol will be developed by adopting current industry practices for production scale manufacturing to optimize expression yields. A column chromatography purification protocol will be developed using modifications to existing purification methods currently used in natural collagenase production. This process will be scaled for production of highly pure recombinant enzymes for use in islet isolation procedures. Finally, an assessment of the different molecular forms and their role in islet isolations will be determined in both human and porcine tissue both internally and at leading islet isolation centers. Using a standard modified Ricordi islet isolation procedure, the specific molecular form will be correlated to successful islet yield using design of experiment statistical control methods. PUBLIC HEALTH RELEVANCE: Pancreatic islet transplantation represents a promising therapy for Type 1 Diabetic patients as a replacement for traditional insulin therapies. Hurdles to moving this therapy to routine clinical practice lies in achieving greater islet yields for either h uman allotransplantation or porcine xenotransplantation. VitaCyte is working to develop a robust and reproducible manufacturing process using recombinant tissue dissociation enzymes to improve islet isolation procedure yields.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government