PEI-somes and cationization of protein probes for microimaging of nervous system

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$361,776.00
Award Year:
2009
Program:
SBIR
Phase:
Phase I
Contract:
1R43NS064614-01A1
Award Id:
94049
Agency Tracking Number:
NS064614
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
VIVID TECHNOLOGIES, 5607 CAREW ST, HOUSTON, TX, 77096
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
198051646
Principal Investigator:
BENXIAO ZHANG
() -
Business Contact:
VLADIMIR DIDENKO
() -
vdidenko@bcm.edu
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): PEI-somes and cationization of protein probes for microimaging of nervous system In this proposal we will develop a technology and a carrier for the delivery of fluorescently labeled fully functional antibodies into liv e cells. This will make possible immunohistochemical reactions inside live cells, eliminating the need for their fixation and processing. The objective is to advance microimaging of nervous system cells. Our approach will be based on a cationic polymer pol yethyleneimine (PEI). We will develop a technology for the production of highly dispersed complexes of this cationic polymer with fluorescently-labeled antibodies, which will self-assemble into liquid particles with particle sizes below 200 nm (PEI-somes). These non-liposomal, non-micelle-based particles will be used for transmembrane delivery of functional antibodies. Application of PEI-somes as transmembrane carriers of fluorescently labeled antibodies for live cell immunohistochemistry will be developed into a marketable product for the needs of neuroscience and biomedical research. Although synthetic cationic polymers such as PEI are efficient DNA carriers, they were not used before as protein carriers. In our preliminary work we were able to assure stro ng complex formation and efficient delivery of functional proteins into cells. The specific aims of this project are: Aim 1: To develop the new enabling technology for the transmembrane transportation of proteins using their reversible cationization via fo rmation of PEI-some particles. Demonstrate intracellular delivery of fluorescently labeled antibodies into live cells and their specific reactions with internal molecular targets, observable through two modalities - fluorescent microscopy and spectroscopy. To test the strength of the PEI-antibody complexes, their chemical properties and specific reactivity. Aim 2: To develop an application of cationized antibodies for the real-time microimaging of nervous system cells. To assess and optimize the effectivene ss of transmembrane delivery of fluorescently tagged antibodies, their intracellular dynamics, toxicity and specific reactivity our preliminary data indicate superior qualities of the polymer-based protein carriers over the currently used peptide- and lipi d-based protein delivery agents. Our project fills the gap in the field of protein transporters by developing a new antibody carrier. The particle-directed intracellular imaging will provide a technological advantage over currently used fixed-cell approach es. It will simplify detection and monitoring of intracellular processes and will also provide a method to monitor both toxicity and efficacy of treatment. The commercial potential for improved imaging agents is exceptional, as these agents are widely used in neuroscience and biomedical research. PUBLIC HEALTH RELEVANCE: The proposed project will result in the development of a new assay for the needs of neuroscience and biomedical research. This technology will advance microimaging of nervous system cells a nd enable live-cell real-time imaging of cellular processes.

* information listed above is at the time of submission.

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