Conditional Mutants of Streptavidin

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
1995
Program:
SBIR
Phase:
Phase I
Contract:
1 R43 CA68821-01,
Agency Tracking Number:
29246
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Western Research Company, Inc.
2127 E Speedway, Suite 209, Tucson, AZ, 85719
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Brian Craine
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
We will isolate a temperature sensitive mutant of streptavidin that will bind biotin at 4C but notbe accomplished by chemical mutagenesis to saturate the streptavidin gene with mutations. A scheme ftemperature sensitive mutant will be employed. The library of mutants and the enriched library willcomputer assisted system for colony screening to find candidate mutants. These mutants will be chararesulting mutants are expected to provide a valuable and novel addition to avidin-biotin technology.

* information listed above is at the time of submission.

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