FAMILY OF LOW-CALORIC SWEETENERS--3-D STRUCTURES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$434,562.00
Award Year:
1991
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
11202
Agency Tracking Number:
11202
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1545 17th St, Santa Monica, CA, 90404
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
(310) 829-7681
Business Contact:
() -
Research Institute:
n/a
Abstract
THE THAUMATINS ARE A FAMILY OF NATURALLY OCCURRING PLANT-DERIVED PROTEINS WITH A SWEET TASTE MANY THOUSANDFOLD MORE INTENSE THAN SUGAR. THESE PROTEINS ARE GENERALLY REGARDED AS SAFE, ARE APPROVED FOR USE AS FOOD ADDITIVES IN NUMEROUS COUNTRIES, DO NOT PROMOTE WEIGHT GAIN, AND HAVE NOTBEEN IMPLICATED IN THE FORMATION OF DENTAL CARIES. XOMA CORPORATION HAS PURIFIED THE TWO MAJOR SPECIES OF PLANT THAUMATIN. DIFFRACTION-GRADE CRYSTALS HAVE BEEN GROWNFOR BOTH PROTEINS, AND REFLECTION DATA ARE BEING COLLECTED. SEVERAL HEAVY ATOM DERIVATIVES HAVE ALREADY BEEN GENERATED. IN ADDITION TO THE NATIVE THAUMATINS THAT WILL FORM THE BASIS FOR CRYSTALLOGRAPHIC ANALYSES, A LIBRARY OF THAUMATIN VARIANTS HAS BEEN PRODUCED BY USING ALTERED SYNTHETIC GENES EXPRESSED VIA A YEAST SECRETION SYSTEM. EACH OF THESE HAS BEEN PURIFIED AND CATEGORIZED AS TO STABILITY AND TASTE CHARACTER. DURING THE INITIAL PHASE OF THIS SUDY, THE STRUCTURE OF THE TWO MAJOR PLANT PROTEINS WILL BE DETERMINED. LATER STUDIES WILL COMPARE THESE TO THE THREE-DIMENSIONAL STRUCTURE OF THAUMATIN DERIVATIVES HAVING ALTERED TASTE PROPERTIES. THE DATE WILL BE USED TO MAP THE THAUMATIN DOMAINS THAT AFFECT TASTE AND THE QUALITY OF SWEETNESS PERCEPTION. BASED ON THESE STRUCTURAL ANALYSES, ATTEMPTS WILL BE MADE TO IDENTIFY OR FURTHER DESIGN SAFE, LOW-CALORIE, NONCARIOGENIC SWEETENERS WITH SPECIFIC TASTE AND STABILITY CHARACTERISTICS.

* information listed above is at the time of submission.

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