Rapid Detection of Listeria Monocytogenes

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: 99-33610-7516
Agency Tracking Number: 1999-00307
Amount: $65,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1999
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Xtrana, Inc.
717 Yosemite Circle, Denver, CO, 80230
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Jeffrey Marmaro
 (303) 363-2465
 jmarmaro@mi-mail.com
Business Contact
 John Wheeler
Title: President and CEO
Phone: (303) 363-2445
Email: jwheeler@mi-mail.com
Research Institution
N/A
Abstract
Not Available Listeria monocytogenes is a widely distributed food pathogen that has recently been associated with a number of food recalls and outbreaks of illness. Xtrana, Inc. is developing a low complexity genetic- based assay for sensitive and specific detection of L. monocytogenes. The successful development and validation of our assay will provide for confirmed detection of Listeria monocytogenes with vastly improved turnaround times. We anticipate that following minimal enrichment times, the assay will be run directly without the usual selective culture and subculture. In Phase I feasibility of extraction of RNA, capture onto a solid phase, isothermal amplification, and visual result detection was demonstrated. We are now ready to proceed with more rigorous sensitivity and specificity confirmation, interface with all of the food types required for AOAC approval, and confirmation that the assay will be specific to only viable Listeria monocytogenes.

* information listed above is at the time of submission.

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