EXPRESSION OF HUMAN PROTEIN C IN MAMMALIAN CELLS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 5009
Amount: $279,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1987
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
2121 N 35th Street, Seattle, WA, 98103
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Mark J Murray
 Principal Investigator
 (206) 632-4036
Business Contact
Phone: () -
Research Institution
N/A
Abstract
A FULL-LENGTH CODING SEQUENCE FOR HUMAN PROTEIN C WILL BE CLONED. THIS WILL BE DONE EITHER BY OBTAINING A 5' END CLONE FROM A LIVER CDNA LIBRARY OR BY FUSING A GENOMIC DNA SEQUENCE TO CDNA, WHICH LACKS AN AMINOTERMINAL CODING SEQUENCE. ONCE THIS CLONE IS COMPLETE IS WILL BE INTRODUCEDINTO AN EXPRESSION VECTOR DESIGNED FOR HIGH-LEVEL EXPRESSIONIN MAMMALIAN CELLS. THIS CONSTRUCTION WILL BE TRANSFECTED INTO BHK CELLS AND EXPRESSED PROTEIN C ACTIVITY MEASURED. PROTEIN C WILL BE ASSAYED BY ELISA AND FOR ANTICOAGULANT ACTIVITY.

* Information listed above is at the time of submission. *

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