EXPRESSION OF HUMAN PROTEIN C IN MAMMALIAN CELLS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$279,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
5009
Agency Tracking Number:
5009
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Zymogenetics Inc. (Currently Zymogenetics Inc)
2121 N 35th Street, Seattle, WA, 98103
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Mark J Murray
Principal Investigator
(206) 632-4036
Business Contact:
() -
Research Institution:
n/a
Abstract
A FULL-LENGTH CODING SEQUENCE FOR HUMAN PROTEIN C WILL BE CLONED. THIS WILL BE DONE EITHER BY OBTAINING A 5' END CLONE FROM A LIVER CDNA LIBRARY OR BY FUSING A GENOMIC DNA SEQUENCE TO CDNA, WHICH LACKS AN AMINOTERMINAL CODING SEQUENCE. ONCE THIS CLONE IS COMPLETE IS WILL BE INTRODUCEDINTO AN EXPRESSION VECTOR DESIGNED FOR HIGH-LEVEL EXPRESSIONIN MAMMALIAN CELLS. THIS CONSTRUCTION WILL BE TRANSFECTED INTO BHK CELLS AND EXPRESSED PROTEIN C ACTIVITY MEASURED. PROTEIN C WILL BE ASSAYED BY ELISA AND FOR ANTICOAGULANT ACTIVITY.

* information listed above is at the time of submission.

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