CLONING, SEQUENCING, AND EXPRESSION OF HUMAN BSA LIPASE CDNA

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
7232
Agency Tracking Number:
7232
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
2121 North 35th Street, Seattle, WA, 98103
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JOHN M MARAGANORE PHD
(206) 632-4036
Business Contact:
() -
Research Institute:
n/a
Abstract
BILE SALT-ACTIVATED LIPASE (BSAL) IS FOUND IN THE MILK OF A LIMITED NUMBER OF MAMMALS: HUMANS, GORILLAS, DOGS, AND CATS THE ENZYME PLAYS AN IMPORTANT ROLE IN THE INFANT DIET IN THEDIGESTION OF LIPIDS, CAUSING A STRIKING QUANTITATIVE DIF- FERENCE BETWEEN BREAST-FED AND FORMULA-FED INFANTS IN WEIGHTGAIN. IN THIS REGARD, IT IS IMPORTANT TO NOTE THAT BSAL IS ABSENT IN BOVINE MILK. IN PHASE I, ZYMOGENETICS, INC, PLANS TO CLONE, SEQUENCE, ANDEXPRESS THE CDNA FOR HUMAN BSAL. THIS WILL PROVIDE A FRAME WORK FOR PHASE II RESEARCH, WHEREIN RECOMBINANT HUMAN BSAL WILL BE PRODUCED IN LARGE QUANTITIES FOR SUPPLEMENTATION IN INFANT FORMULAS. EXPRESSION OF THE HUMAN BSAL CDNA WILL BE ACHIEVED BY CLONING INTO AN EXPRESSION VECTOR SUITABLE FOR PRODUCTION AT HIGH LEVELS IN SACCHAROMYCES CEREVISIAE. LEVELS OF EXPRESSION WILL BE ASCERTAINED BY ELISA AND DIRECTMEASUREMENTS OF ENZYMATIC ACTIVITY. THE RECOMBINANT PROTEINWILL BE PURIFIED AND CHARACTERIZED FOR ITS PHYSICAL AND CHEMICAL SIMILARITY TO THE PURIFIED HUMAN MILK ENZYME

* information listed above is at the time of submission.

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