LOW-COST HELPER-CELL MONITORING USING NOVEL CELL LINKERS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1990
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
13659
Agency Tracking Number:
13659
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
371 Phoenixville Pke, Malvern, PA, 19355
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Katharine Muirhead
(215) 889-2206
Business Contact:
() -
Research Institution:
n/a
Abstract
QUANTITATION OF CD4(+) LYMPHOCYTE LEVELS BY FLOW CYTOMETRY IS THE MOST COMMON MEASURE OF PROGRESSION OF HIV-INFECTED INDIVIDUALS TOWARD FRANK AIDS. THIS PROJECT WILL COUPLE NOVEL LIPOPHILIC CELL LINKERS WITH EXISTING MAGNETIC BEAD-BASED CELL SEPARATION TECHNIQUES TO PRODUCE AN INEXPENSIVE ALTERNATIVE SUITABLE FOR ROUTINE CD4(+) LYMPHOCYTE MONITORING, ALLOWING FCM RESOURCES TO BE DIRECTEDTOWARD OTHER TESTS REQUIRING MORE OPERATOR EXPERTISE. PRELIMINARY STUDIES USING FRESH MONONUCLEAR CELL PREPARATIONS FROM NORMAL INDIVIDUALS SHOW HIGH CORRELATION BETWEEN THIS ASSAY AND FLOW CYTOMETRY. PHASE I GOALS ARE DIRECTED TOWARD ASSAY SIMPLIFICATION AND VALIDATION ESSENTIAL TO DEVELOPMENT OF A CLINICALLY USEFUL IN VITRO DIAGNOSTIC TEST. GOALS INCLUDE: (1) DISCRIMINATION BETWEEN CD4(+) LYMPHOCYTES AND MONOCYTES; (2) SIMPLIFICATION OF SPECIMEN PREPARATION; (3) REDUCTION OF HIV-RELATED BIOHAZARDS; AND (4) EVALUATION OF THE RESULTING ASSAY IN WHOLE BLOOD SAMPLES HAVING A WIDE RANGE OF CD4(+) LYMPHOCYTELEVELS, OBTAINED FROM INDIVIDUALS AT DIFFERENT STAGES OF HIV INFECTION AND TREATMENT. A MEDICAL TECHNOLOGIST SHOULD BE ABLE TO IMPLEMENT THE FINAL PROCEDURE IN 1 TO 2 DAYS, MAKING IT SUITABLE FOR COMMUNITY HOSPITALS, LARGE CLINICS, AND THIRD-WORLD SETTINGS.

* information listed above is at the time of submission.

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