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Fused-core Particles for Ultra-fast Proteomic and Genomic Separations

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44GM077688-02
Agency Tracking Number: GM077688
Amount: $675,000.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: PHS2007-2
Timeline
Solicitation Year: 2008
Award Year: 2008
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
ADVANCED MATERIALS TECHNOLOGY, INC. 3521 SILVERSIDE ROAD, SUITE 1-K, QUILLEN BLDG
WILMINGTON, DE 19810
United States
DUNS: 557435273
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 () -
Business Contact
Phone: (302) 477-2513
Research Institution
N/A
Abstract

DESCRIPTION (provided by applicant): This project proposes unique column packings for the fast high-performance HPLC of biomacromolecules. Initial focus will be on developing techniques to modify the chemistry of silica surfaces using hydrothermal methods,
so that biomolecular separations are optimized for efficiency, peak shape and compound yields. Second, three new particle types will be synthesized and tested, with two of the new particles optimized for the rapid separation of peptides and proteins, resp
ectively. The third new particle type is for the high-speed separation of larger biomolecular weight components by size-exclusion (gel filtration) liquid chromatography. In Phase 1 grant, 1R43GM077688-01, we developed unique fused-core silica microsphere
s using new nanoparticle technology. These particles are prepared by a proprietary multi- multilayering method that permits the fabrication of strong porous outer shells on solid silica cores. The outer shells can be made with different thicknesses and por
e sizes, depending on intended application. Narrow-pore fused-core particles recently were commercialized to produce HaloTM columns. These columns demonstrate very fast separations of pharmaceutical and small biological compounds because of unusually high
chromatographic efficiency and excellent mass transfer (kinetic) properties. Because of an extremely narrow particle size distribution and higher particle density, packed columns of fused-core particles demonstrate superior efficiency and unusual stability
at high mobile phase flow rates and column inlet pressures. The proposed program will synthesize new wide-pore particles that have the pore size, particle size, outer shell thickness and surface chemistry optimized for the rapid separation of peptides, pr
oteins, DNA fragments, and other higher molecular weight biomacromolecules. These new fused-core particles will be superior for proteomic and genetic studies, especially when multidimensional ( 2-D ) separations are needed, and are highly suited for HPLC/M
S bioscience applications. The new materials proposed in this project for rapid high-resolution liquid chromatographic separations will strongly enhance research efforts in proteomics, DNA-based studies and related bioscience applications. The incre
ased capability for rapid characterizations and measurements will assist in the search for defining the cause and cure of disease-related problems, help in developing new drugs and support projects to improve the environment and general health.

* Information listed above is at the time of submission. *

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