Nanofilament Silicon for Ultrasensitive Proteomics

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$400,002.00
Award Year:
2008
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM084525-01
Agency Tracking Number:
GM084525
Solicitation Year:
2008
Solicitation Topic Code:
n/a
Solicitation Number:
PHS2007-2
Small Business Information
IO BIOSYSTEMS, INC.
IO BIOSYSTEMS, INC., 5619 SONOMA RD, BETHESDA, MD, 20817
Hubzone Owned:
Y
Socially and Economically Disadvantaged:
Y
Woman Owned:
Y
Duns:
802266671
Principal Investigator:
() -
Business Contact:
(301) 240-4379
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): A novel platform for ultrasensitive and matrix-free laser desorption/ionization mass spectrometry (LDI-MS) based on nanofilament silicon surfaces is proposed. Currently, the sensitivity and overall functionality of matr ix-free LDI-MS performed on porous silicon (pSi) chips are limited by a number of factors imposed by standard pSi substrates. In this proposal, we seek to address these issues through the development of novel silicon nanofilament substrates fabricated usin g metal-assisted oxidative etching and reactive-ion etching, with electrowetting used to provide dynamic control over surface hydrophobicity. This feature will allow switching of the surfaces from a superhydrophobic state to a strongly hydrophilic state, a llowing for complete wetting of the nanofilaments for optimal sample deposition while limiting sample dispersion and providing a method to achieve on-target sample concentration, thereby enabling routine detection limits of 10-100 zmol (10-20 to 10-19 mol) for complex peptide samples. PUBLIC HEALTH RELEVANCE: The analysis of proteins from biological samples is enabling advances in the treatment and diagnosis of disease. This project addresses the development of an ultrasensitive nanotechnology-based platform which can improve the ability to perform protein analysis from limited samples such as tissue biopsies.

* information listed above is at the time of submission.

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