Development of High Throughput Molecular Profiles for the Detection and Staging of Cancer

Award Information
Agency: Department of Defense
Branch: Army
Contract: DAMD17-03-C-003
Agency Tracking Number: A022-0079
Amount: $119,806.00
Phase: Phase I
Program: SBIR
Awards Year: 2003
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
1015 Grandview Drive, South San Francisco, CA, 94080
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Dongxiao Zhang
 VP of Genomics
 (650) 583-6688
Business Contact
 Guo-Liang Yu
Title: President and CEO
Phone: (650) 583-6688
Research Institution
This Small Business Innovation Research Phase I project aims to develop a technology that employs polyclonal antibodies as a complex probe to discover tumor associated surface antigens, a process named Dual Immunostaining-mediated Subtractive Cloning(DISC). Antibodies have been the most powerful tools to dissect the expression and structure of proteins. Immune system has evolved to respond to foreign antigens by generating antibodies recognizing most, if not all, foreign epitopes. It is thus apowerful tool to search for structural and quantitative differences on cell surface proteins between a normal cell and a diseased cell. Disease-associated cell surface antigens are ideal markers for diagnostics and potential targets for therapeuticintervention. To discover novel disease-related cell surface molecules, we propose a novel technology that combines three proven techniques: whole cell immunization, flow cytometry and cDNA expression cloning, to identify surface antigens thatquantitatively or structurally unique to diseased cells such as cancer cells. These tumor-associated antigens identified in this process will be used to generate monoclonal antibody microarrays for the detection and staging of cancer in Phase II of theproject. DISC technology that Epitomics is attempting to develop in Phase I will lead to discovery of novel tumor associated antigens in a panel of human cancers (Phase II project). Rabbit monoclonal antibodies against these antigens will be producedfrom the cell lines generated in DISC process. Epitomics intends to develop and commercialize antibody microarrays for cancer detection and staging, using monoclonal antibodies for the existing and newly discovered tumor antigens.

* Information listed above is at the time of submission. *

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