Amplification of Proteins in Body Fluids for Early Detection of Biological Warfare Exposure

Award Information
Agency:
Department of Defense
Amount:
$69,916.00
Program:
SBIR
Contract:
W81XWH-04-C-0033
Solitcitation Year:
2003
Solicitation Number:
2003.2
Branch:
Army
Award Year:
2004
Phase:
Phase I
Agency Tracking Number:
A032-1664
Solicitation Topic Code:
A03-173
Small Business Information
EPITOMICS, INC.
1015 Grandview Drive, South San Francisco, CA, 94080
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
118926596
Principal Investigator
 Dongxiao Zhang
 Vice President of Genomic
 (650) 583-6688
 dongxiao.zhang@epitomics.com
Business Contact
 Guo-Liang Yu
Title: President and CEO
Phone: (650) 583-6688
Email: guoliang.yu@epitomics.com
Research Institution
N/A
Abstract
This SBIR Phase I project will lead to the development of a technology to detect very low levels of multiple pathogen-specific proteins or human proteins very quickly after exposure to biological warfare (BW) agents. The technology will link several proven techniques in a unique sequence so that a mixture of biological warfare proteins can be accurately and sensitively detected in one simple procedure. First, we will generate high affinity rabbit monoclonal antibody (RabMAb) pairs for specific biomarkers, using our proprietary rabbit hybridoma technology. Second, we will develop a sensitive, multiplexed assay system that combines antibody binding, T7 RNA polymerase amplification and DNA array detection. Our technology will eliminate the requirement of sophisticated proteomic instruments, radioactive labeling and large scale automation, yet will possess the potential to detect hundreds of low level proteins in a simple assay. We have named the novel protein detection technology Multiplexed Quantification Using Antibodies and T7-amplification, or MultiQUANT. In Phase I, we will generate RabMAb pairs for 3 BW biomarkers. Concomitantly, we will validate MultiQUANT technology using anti-fibronectin RabMAb pairs that we already have in our possession. In Phase II, we will develop the MultiQUANT system for several dozen BW biomarkers.

* information listed above is at the time of submission.

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