IT IS THE OVERALL OBJECTIVE OF THIS PROJECT TO DEVELOP A TELEOST FRY EXPLANT PROCEDURE AS A SIMPLE, SENSITIVE, RAPID METHOD OF EVALUATING AQUATIC POLLUTANTS.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1984
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
1442
Agency Tracking Number:
1442
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Southern Station, P.o. Box 10072, Hattieburg, MS, 39406
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
PAULA GREGORY
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
IT IS THE OVERALL OBJECTIVE OF THIS PROJECT TO DEVELOP A TELEOST FRY EXPLANT PROCEDURE AS A SIMPLE, SENSITIVE, RAPID METHOD OF EVALUATING AQUATIC POLLUTANTS. PHASE I WILL DETERMINE THE FEASIBILITY OF SUCH A SYSTEM BY EVALUATING THE TECHNIQUE WITH A DIRECT ACTING PROCARCINOGEN (BENZIDINE HYDROCHLORIDE) AND A MUTAGEN (MITOMYCIN C.) PHASE I EXPERIMENTS WILL UTILIZE TWO TELEOST SYSTEMS -- THE SHEEPHEAD MINNOW (CYPRINODON VARIEGATUS) AND THE RAINBOW TROUT (SALMO GAIRDNERI). EXPOSED EXPLANTS WILL BE EXAMINED MORPHOLOGICALLY, CYTOGENETICALLY (I.E. CHROMOSOME BANDING, SISTER-CHROMATID EXCHANGE) AND EVALUATED FOR THEIR ABILITY TO FORM COLONIES. IN ADDITION, THE MIXED FUNCTION OXIDIZED SYSTEM OF THE EXPLANTS WILL BE EVALUATED. IN PHASE II, IT IS OUR INTENT TO PRODUCE MONOCLONAL ANTIBODIES RECOGNIZED ALL SURFACE ANTIGENS OF TRANSFERRED CELLS, AND TO USE THESE ANTIBODIES AS REAGENTS AS EARLY INDICATIONS OF CELL TRANSFORMATIONS IN THE TELEOST EXPLANT SYSTEM.

* information listed above is at the time of submission.

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