Universal Sample Preparation using Microstructured Arrays with Plasma Lysis

Award Information
Agency: Department of Defense
Branch: Defense Advanced Research Projects Agency
Contract: W31P4Q-09-C-0126
Agency Tracking Number: 08SB2-0302
Amount: $98,820.00
Phase: Phase I
Program: SBIR
Awards Year: 2008
Solicitation Year: 2008
Solicitation Topic Code: SB082-012
Solicitation Number: 2008.2
Small Business Information
600 SE Assembly Ave., Bldg. 55 Suite 100, Vancouver, WA, 98661
DUNS: 191088892
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Joseph Birmingham
 Chief Technical Officer
 (360) 694-3704
 joseph.birmingham@microsti.com
Business Contact
 Joseph Birmingham
Title: President
Phone: (360) 694-3704
Email: joseph.birmingham@microsti.com
Research Institution
N/A
Abstract
DARPA is seeking an universal sample preparation methods for extraction of nucleic acid capable of being used in many different assay systems for subsequent detection. The method needs to produce nucleic acids from spores, viruses, bacteria (or vegetative cells) that are ready for a wide variety of diagnostic equipment. MicroStructure Technologies (MicroST) in conjunction with Oregon Health and Science University (OHSU) has demonstrated a two-step nucleic acid production method. The first step is the efficient retention and selective concentration of biomaterials from liquid or bioaerosol directly onto coated microstructured arrays. After capture of the biomaterials onto the micropillars, the next step is an ionized gas (or plasma) treatment for about a minute to extract nucleic acids. The easy-to-use approach can be automated. The plasma lysates produced have been demonstrated to be ready for PCR without any additional processing. The rapid nucleic acid preparation method consumes few watts for biomaterial collection (from air or liquid) onto the microstructured arrays and plasma lysing. The relevance of this effort is to rapidly provide nucleic acids for biodetector applications in a miniature package with minimal logistics burden. A critical need exists for a universal sample preparation method that relies on little power to produce the nucleic acids for any biodetector.

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