Aptamer-Based Analysis and Extraction of Sitosterol and Related Compounds

Award Information
Agency: Department of Energy
Branch: N/A
Contract: DE-FG02-02ER83510
Agency Tracking Number: 70846B02-I
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
1111 North Hyland, Ames, IA, 50014
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Richard Hamilton
 (515) 292-5141
Business Contact
 Linda Lyngholm
Title: 70846
Phone: (515) 292-5141
Email: molexpress@msn.com
Research Institution
70846 Oilseed contains sterols and related compounds with economic potential. The extraction and analysis of these compounds would be aided by the availability of highly selective aptamers with high affinities for their particular sterol ligands. This project will develop aptamers for use in microarrays to analyze and extract sterol contents of oil and other biological extracts. Bacterial expression vectors will be prepared from which the aptamers can be expressed in large quantities. In Phase I, one or more DNA aptamers for sitosterol will be selected. The aptamers will be evaluated for their specificity and affinity for sitosterol and related compounds. A bacterial expression vector will be developed from which aptamers can be prepared. Commercial Applications and Other Benefits as described by the awardee: Commercial applications include (1) the use of aptamers in the analysis and extraction of sitosterol and related compounds, and (2) a general method for economically mass-producing DNA aptamers for use in many analytical procedures.

* Information listed above is at the time of submission. *

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