FP-Aptamer Reader for Algal Toxins

Award Information
Agency:
Department of Commerce
Branch
n/a
Amount:
$49,989.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
DG1330-02-CN-0039
Award Id:
57290
Agency Tracking Number:
2002-23
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
101 West Sixth Street, Suite 200, Austin, TX, 78701
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JohnBruno
Chief Technical Officer
(512) 479-7732
bruno@spec.com
Business Contact:
JamesMcNeal
Contracts Director
(512) 479-7732
mcneal@spec.com
Research Institute:
n/a
Abstract
OmniSite BioDiagnostics (OmniSite) proposes to develop artificial receptors composed of DNA oligomers (called ¿Aptamers") for binding and detecting algal toxins. DNA aptamers can be mass produced with relative ease compared to antibody production and purification. The aptamers will be 5'-Cy 5 fluorochrome labeled and used in simple one-step binding fluorescence polarization (FP) assays that do not require wash steps. In Phase 1, OmniSite proposes to generate a set of specific fluoresceinated DNA aptamers to bind and detect Brevetoxin and Saxitoxin. Detection of the aptamer bound toxins will be accomplished via an FP breadboard system to be designed and constructed by OmniSite. In Phase 2, OmniSite will produce a hand-held FP prototype detector with wireless Internet connectivity so that test results can be accessed remotely. In addition, OmniSite will clone and sequence the anti-toxin aptamers and optimize FP aptamer assays for field use by water and seafood inspectors

* information listed above is at the time of submission.

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