Fluoresent Signaling Aptamers for Rodent B&T Cell Analysis

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$69,998.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
DAMD17-02-C-0039
Award Id:
58147
Agency Tracking Number:
A012-1693
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
101 West Sixth Street, Suite 200, Austin, TX, 78701
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
001021042
Principal Investigator:
John Bruno
Chief Technical Officer
(512) 479-7732
bruno@spec.com
Business Contact:
Donald Gorsuch
President
(512) 479-7732
gorsuch@spec.com
Research Institution:
n/a
Abstract
"OmniSite BioDiagnostics proposes to demonstrate and develop artificial receptors composed of DNA called "aptamers" to replace anti-B and T lymphocyte and cytokine antibodies for rabbit and guinea pig immunologic analyses. DNA aptamers will be generated bythe Systematic Evolution of Ligands by EXponential enrichment (SELEX) combinatorial chemistry technique. The anti-rodent B and T cell DNA aptamers will be engineered with intrachain fluorochrome and quencher pairs that will cause a change in fluorescenceintensity upon binding to target lymphocyte subpopulations or cytokines. Hence, such aptamers will be useful in fluorescence microscopy, flow cytometric, and spectrofluorometric analyses. SELEX aptamers offer several major advantages over antibodytechnology; most notably obviating the use of animal hosts and taking advantage of tremendous sequence diversity to select very high affinity specific binding DNA molecules developed against any target molecule. SELEX DNA aptamers can be rapidly andinexpensively mass produced with relative ease compared to antibody production and purification.In Phase I, OmniSite proposes to develop a set of specific DNA aptamers to characterize rabbit and guinea pig memory B and T cells as well as key cytokines. In Phase II, the anti-cellular and anti-cytokine aptamers will be cloned and sequenced and theirfluorescence properties will be optimized for use in flow cytometry. There is a worldwide need for

* information listed above is at the time of submission.

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