Handheld FP - Gene Microarray Reader for Assessment of Radiation Damage

Award Information
Agency:
National Aeronautics and Space Administration
Branch
n/a
Amount:
$69,995.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
NAS3-02020
Agency Tracking Number:
013793
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
OmniSite BioDiagnostics Inc.
101 W. Sixth Street, Suite 200, Austin, TX, 78701
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
John Bruno,
Principal Investigator
(512) 479-7732
bruno@spec.com
Business Contact:
Syd Ulvick,
CTO & VP Development
(512) 479-7732
ulvick@spec.com
Research Institution:
n/a
Abstract
Omnisite BioDiagnostics (OmniSite) proposes to develop a handheld fluorescence polarization (FP) reader for genetic microarrays to assess transcriptional changes in the genes of radiation-exposed astronauts. The reader will be capable of extracting messenger RNA (mRNA) from the blood or other tissues of an astronaut and looking for changes in the transcription of radiation-associated genes such as p53 on commercially available microscope slide-based gene arrays. Amplification of the RNA will be performed either by a variation of Rolling Circle Amplification (RCA) or Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The advantage of using the FP mode of detection on the microarray is obviating the need for wash steps, thus making assays simpler and faster. FP enables homogenous one-step assays without sacrificing sensitivity.In Phase I, OmniSite will provide proof-of-concept for the detection of mRNAs on a gene array using an FP breadboard setup. OmniSite will also provide design parameters for a miniaturized handheld FP reader. In phase II, OmniSite will construct the handheld FP reader and demonstrate its use on real animal blood samples.

* information listed above is at the time of submission.

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