EXPRESSION--SYNTHETIC DNA SEQUENCES/HTLV-I ENV PROTEN

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
11440
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Amgen
1900 Oak Terrace Ln, Thousand Oaks, CA, 91320
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
HUMAN T-CELL LEUKEMIA VIRUS I (HTLV-I) IS A TRANSFORMING RETROVIRUS ETIOLOGICALLY ASSOCIATED WITH ADULT T-CELL LEUKEMIA/LYMPHOMA AND TROPICAL SPASTIC PARAPARESIS. THE NUMBER OF HTLV-I SEROPOSITIVE BLOOD DONORS IN THE UNITED STATES IS BECOMING ALARMINGLY HIGH, RAISING CONCERNS ABOUT CONTAMINIATION OF TRANSFUSABLE BLOOD PRODUCTS. DEVELOPMENT OF A HIGHLY SENSITIVE AND HIGHLY SPECIFIC BLOOD-SCREENING ASSAY FOR HTLV-I ANTIBODIES IN SERUM HAS BEEN HINDERED BY LIMITING QUANTITIES OF CERTAIN VIRAL PROTEINS, INCLUDING THE MOST IMMUNOGENIC, WHICH IS ENVELOPE PROTEIN. THE PHASE I STUDY OUTLINES A STRATEGY FOR EXPRESSING SELECTED PROTEINS, AS WELL AS THE ENTIRE ENVELOPE PROTEIN, USING SYNTHETIC DNA CODING SEQUENCES. IT IS ANTICIPATED THAT THIS APPROACH WILL ALLOW THESE POLYPEPTIDES TO BE EXPRESSED AT HIGH LEVELS IN E. COLI. THE EXPRESSED POLYPEPTIDES WILL BE ANALYZED BIOCHEMICALLY AND EVALUATED FOR THEIR ABILITY TO DETECT ANTIBODIES TO HTLV-I IN HUMAN SERA. IN PHASE II, PROMISING CANDIDATE ENVELOPE POLYPEPTIDES WILL BE USED TO CONFIGURE AN ENZYME-LINKED IMMUNOASSAY (EIA) TO DETECT ANTIBODIES TO HTLV-1 IN HUMAN SERA. THE EIA WILL BE EVALUATED FOR SENSITIVITY AND SPECIFICITY USING SERA FROM A LARGE NUMBER OF NORMAL AND INFECTED INDIVIDUALS. IT IS ENVISAGED THAT A COMPLEMENTARY SET OF RECOMBINANT-DERIVED HTLV-I PROTEINS WILL BE CONFIGURED INTO A HIGH-QUALITY BLOOD-SCREENING ASSAY FOR EXPOSURE TO THIS VIRUS.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government