SBIR Phase II: Microbial Source Tracking Using Mitochondrial DNA for Identification of Contaminant Sources

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: 1056963
Agency Tracking Number: 1056963
Amount: $499,999.00
Phase: Phase II
Program: SBIR
Awards Year: 2011
Solicitation Year: 2011
Solicitation Topic Code: Phase II
Solicitation Number: N/A
Small Business Information
MICROBIAL INSIGHTS INC
2340 STOCK CREEK BLVD, ROCKFORD, TN, 37853-3304
DUNS: 858462625
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Brett Baldwin
 (865) 573-8188
 bbaldwin@microbe.com
Business Contact
 Brett Baldwin
Title: DEng
Phone: (865) 573-8188
Email: bbaldwin@microbe.com
Research Institution
 Stub
Abstract
This Small Business Innovation Research (SBIR) Phase II project will result in field validated microbial source tracking (MST) assays that provide cost-effective identification of sources of fecal pollution. Despite efforts mandated under the BEACH and Clean Water Acts, beach closures have exceeded 20,000 days in each of the last four years primarily due to fecal pollution. The problem continues because traditional methods cannot identify the sources of fecal contamination (sewage, livestock, domestic animals, wildlife). MST assays employing quantitative polymerase chain reaction (qPCR) were developed to quantify source-specific genetic markers encoded on the mitochondrial DNA (mtDNA) of the source animal (human, cattle, dog, etc.). The Phase I results demonstrated that mtDNA-based assays combined with bacterial source tracking methods will provide conclusive identification of fecal contamination sources allowing implementation of corrective measures to improve water quality and protect human health. Phase II studies will include a modification of the DNA extraction procedure to permit quantification of live fecal bacteria to aid in risk assessment and extended field validation studies at two beaches and two coastal watersheds impaired by unknown sources of fecal pollution. The broader impacts of this research are that the MST assays developed and validated during the Phase II project will empower stakeholders with the type of actionable data required to identify fecal contamination sources, implement appropriate corrective actions, and safeguard the nation?s waters. Fecal contamination of water resources currently exacts a severe toll in terms of increased risks to human health and impacts on coastal economies.

* information listed above is at the time of submission.

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