Microfluidic DNA Quality Control Solutions for Biobanks

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43HG005796-01A1
Agency Tracking Number: R43HG005796
Amount: $152,084.00
Phase: Phase I
Program: SBIR
Awards Year: 2011
Solicitation Year: 2011
Solicitation Topic Code: NHGRI
Solicitation Number: PA10-050
Small Business Information
ADVANCED LIQUID LOGIC
615 Davis Dr., Suite 800, RESEARCH TRIANGLE PARK, NC, -
DUNS: 140695474
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 ZHISHAN HUA
 (919) 287-9010
 zhua@liquid-logic.com
Business Contact
 RICHARD WEST
Phone: (919) 287-9010
Email: rwest@liquid-logic.com
Research Institution
 Stub
Abstract
DESCRIPTION (provided by applicant): Recent technological advances in genotyping and DNA sequencing platforms have enabled ever larger and more ambitious projects aimed at understanding the genetic basis of human disease. Specialized biobanks have emergedto meet the needs of these studies by providing for the centralized collection, storage and management of 100,000's of samples. Maintaining the quality of such large collections requires significant expenditure of time and effort with currently available techniques. The goal of this project is to develop an automated and low-cost DNA quality control solution for use in biobanks. The proposed device will combine droplet-based Digital Microfluidic sample handling and real-time PCR with gel electrophoresis in a single unified disposable cartridge. In addition to performing quantitation and quality assessment by sizing, the device will detect contamination (i.e. bacterial DNA) in the sample by real-time PCR. The aims of the Phase I project are to establish thefeasibility of implementing and then integrating each of these processes in a single device. Subsequent work in Phase II would be focused on automating all aspects of the process on a full prototype and scaling-up the number of samples to enable high-throughput multiplexed operation. PUBLIC HEALTH RELEVANCE: The goal of this project is to develop a quality control device to improve the quality and reduce the cost of large genetic studies using samples provided through biobanks.

* information listed above is at the time of submission.

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