A novel ion source for efficient coupling of liquid chromatography and mass spect

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$200,000.00
Award Year:
2011
Program:
SBIR
Phase:
Phase I
Contract:
1R43RR031942-01
Agency Tracking Number:
R43RR031942
Solicitation Year:
2011
Solicitation Topic Code:
NCRR
Solicitation Number:
PA07-451
Small Business Information
MASSTECH, INC.
6992 COLUMBIA GATEWAY DR, #200, COLUMBIA, MD, -
Hubzone Owned:
N
Socially and Economically Disadvantaged:
Y
Woman Owned:
N
Duns:
123310083
Principal Investigator:
VADYM BERKOUT
(443) 539-1742
vberkout@apmaldi.com
Business Contact:
RICHARD LEE
(443) 539-1742
rlee@apmaldi.com
Research Institution:
Stub




Abstract
DESCRIPTION (provided by applicant): The primary objective of this proposal is to develop a novel ion source for the efficient on-line coupling of a wide array of chromatographic techniques with mass spectrometry. The use of liquid chromatography separation is a prerequisite for high-throughput mass spectrometry analysis of complex proteomics samples. Commonly used electrospray ionization (ESI), although allowing a direct coupling, imposes significant limitations on the mobile phases and additives used in LC separations. The most widely used separation technique, reversed-phase liquid chromatography, is performed in non-optimal conditions to satisfy operational requirements of the electrospray ion source. The proposed ion source will overcome these limitations and provide efficient analyte ionization from a wide range of liquid sample compositions. Thus, the ion source will allow RP-LC to perform in optimal conditions and also the use of chromatographic techniques not previously amenable by direct ESI-MS analysis. PUBLIC HEALTH RELEVANCE: Proteomics studies biological processes by the systematic analysis of proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose anew high-throughput technology for the efficient on- line coupling of a wide array of chromatographic separation techniques with mass spectrometry.

* information listed above is at the time of submission.

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