A novel ion source for efficient coupling of liquid chromatography and mass spect

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$200,000.00
Award Year:
2011
Program:
SBIR
Phase:
Phase I
Contract:
1R43RR031942-01
Award Id:
n/a
Agency Tracking Number:
R43RR031942
Solicitation Year:
2011
Solicitation Topic Code:
NCRR
Solicitation Number:
PA07-451
Small Business Information
6992 COLUMBIA GATEWAY DR, #200, COLUMBIA, MD, -
Hubzone Owned:
N
Minority Owned:
Y
Woman Owned:
N
Duns:
123310083
Principal Investigator:
VADYMBERKOUT
(443) 539-1742
vberkout@apmaldi.com
Business Contact:
RICHARDLEE
(443) 539-1742
rlee@apmaldi.com
Research Institute:
Stub




Abstract
DESCRIPTION (provided by applicant): The primary objective of this proposal is to develop a novel ion source for the efficient on-line coupling of a wide array of chromatographic techniques with mass spectrometry. The use of liquid chromatography separation is a prerequisite for high-throughput mass spectrometry analysis of complex proteomics samples. Commonly used electrospray ionization (ESI), although allowing a direct coupling, imposes significant limitations on the mobile phases and additives used in LC separations. The most widely used separation technique, reversed-phase liquid chromatography, is performed in non-optimal conditions to satisfy operational requirements of the electrospray ion source. The proposed ion source will overcome these limitations and provide efficient analyte ionization from a wide range of liquid sample compositions. Thus, the ion source will allow RP-LC to perform in optimal conditions and also the use of chromatographic techniques not previously amenable by direct ESI-MS analysis. PUBLIC HEALTH RELEVANCE: Proteomics studies biological processes by the systematic analysis of proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose anew high-throughput technology for the efficient on- line coupling of a wide array of chromatographic separation techniques with mass spectrometry.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government