Miniaturized DNA Sequencer for Identification of Microbial Pathogens

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$1,454,210.00
Award Year:
2011
Program:
SBIR
Phase:
Phase II
Contract:
2R44HG005186-02
Agency Tracking Number:
R44HG005186
Solicitation Year:
2011
Solicitation Topic Code:
NHGRI
Solicitation Number:
PA11-096
Small Business Information
ADVANCED LIQUID LOGIC
615 Davis Dr., Suite 800, RESEARCH TRIANGLE PARK, NC, -
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
140695474
Principal Investigator:
MICHAEL POLLACK
(919) 287-9010
mgp@liquid-logic.com
Business Contact:
RICHARD WEST
(919) 287-9010
rwest@liquid-logic.com
Research Institution:
Stub




Abstract
DESCRIPTION (provided by applicant): The ultimate goal of this project is to develop an extremely compact and inexpensive DNA sequence for applications in laboratory and clinical settings. The device will implement pyrosequencing on a disposable droplet based digital microfluidic cartridge and will be capable of sequencing 10's to 100's of nucleotides at a very low cost per run and at a low initial equipment investment. This platform will also integrate sample preparation, including DNA amplification, with sequencing to provide the integration and ease-of-use demanded of clinicians and other non-specialists who require DNA sequence data. The initial application for this capability will be microbial pathogen identification. In Phase I, a prototype was developed and the microfluidic protocols and biochemical reaction conditions for pyrosequencing were optimized. As a feasibility demonstration 20-40 base pairs within the ITS2 genetic region were sequenced for 13 different molds and yeasts which provided unambiguous identification of each isolate. In Phase II, this success will be built upon by integrating sample preparation steps including DNA concentration, DNA amplification, and template preparation into the system. The integrated system will be evaluated andcompared to other commercially available solutions in terms of speed, cost and accuracy of fungal identification. Further optimization of the pyrosequencing biochemistry will be performed to enable longer read lengths on shorter time scales while retaining high accuracy. Finally, a data analysis tool will be developed to automate base calling and database queries. The final product will represent a streamlined approach to fungal species identification, integrating a number of components of the contemporarypathogen identification workflow. PUBLIC HEALTH RELEVANCE: A compact and inexpensive instrument which can automatically identify fungal isolates by sequencing a small region of their DNA will be developed and tested. This product would greatly improve the ability of clinicians to treat infectious disease.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government