Engineered proteases for proteomics

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$1,537,034.00
Award Year:
2011
Program:
SBIR
Phase:
Phase II
Contract:
2R44RR025973-02
Award Id:
n/a
Agency Tracking Number:
R44RR025973
Solicitation Year:
2011
Solicitation Topic Code:
NCRR
Solicitation Number:
PA07-451
Small Business Information
11305 DUNLEITH PL, NORTH POTOMAC, MD, -
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
Y
Duns:
193771347
Principal Investigator:
BIAO RUAN
(301) 610-9687
ruan@umbi.umd.edu
Business Contact:
BONNIE BRYAN
(301) 610-9687
potomac_affinity@msn.com
Research Institution:
Stub




Abstract
DESCRIPTION (provided by applicant): Proteomics is a rapidly expanding field but current methodologies remain inadequate for achieving its full potential. The most basic enzymological tool for characterizing proteins is the protease. Proteases are alreadyan essential part of proteomic analysis but more sophisticated tools are needed to identify low abundance proteins in highly complex samples. We have engineered prototype restriction proteases which are active in denaturing conditions and which cut specifically at well-defined cognate sequence motifs. Our basic innovation would be the ability to parse a proteome into sequence-edited slices and to detect these edited portions with high resolution and high sensitivity. In Phase I we tested the prototype restriction protease for suitability in proteomic analysis and implemented a novel directed evolution methodology for the selection of proteases that cut new sequence motifs. The Phase II objective is to develop a sophisticated set of protease tools to facilitate proteomic analysis in the way restriction endonucleases have facilitated genomic analysis. PUBLIC HEALTH RELEVANCE: The complex and dynamic nature of proteomes make them rich with useful information but difficult to characterize. Our long-rangeobjective is to develop a sophisticated set of protease tools to facilitate proteomic analysis in the way restriction endonucleases have facilitated genomic analysis. Better proteomic tools will lead to earlier detection of disease states, better treatments, better predictability of the effects of various treatments, and the development of individualized therapies.

* information listed above is at the time of submission.

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