DEVELOPMENT OF A RAPID IN VITRO SCREENING ASSAY FOR DETECTION OF CANCER BIOMARKERS IN URINE AND BLOOD.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$200,000.00
Award Year:
2011
Program:
SBIR
Phase:
Phase I
Contract:
N43CO110069
Award Id:
n/a
Agency Tracking Number:
N43CO110069
Solicitation Year:
2011
Solicitation Topic Code:
NCI
Solicitation Number:
n/a
Small Business Information
1310 RESEARCH PK DR, MANHATTAN, KS, 66502-5000
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
946856804
Principal Investigator:
FRANK KROH
(785) 537-0179
MADISONA@NANOSCALECORP.COM
Business Contact:
FRANK KROH
(785) 537-0179
MADISONA@NANOSCALECORP.COM
Research Institution:
n/a
Abstract
The goal of the project is to develop a rapid in vitro screening assay for detection of cancer biomarkers in urine and blood. The aim is testing and optimization of nanoparticle sensors, based on cyanine dyes that are chemically linked to Fe/Fe3O4 nanoparticles via protease-selective consensus (cleavage) sequences. The focus is on the quantitative determination of active cancer-specific proteases in urine and blood via simple fluorescence measurements. The severing from the nanoparticle stops the efficientfluorescence quenching of the organic luminophore by the nanoparticle, thus allowing the detection of the protease activity. The matrix metalloproteinases, urokinase-type plasminogen activator, and cathepsins, are up-regulated in the vast majority of progressing cancers and can, therefore, serve as markers for cell survival/tumor progression, angiogenesis, and tissue remodeling/ invasion. The focus will be on pancreatic cancer, and the studies will be done on urine and blood from 30 patients and 12 healthy individuals. Measuring twelve proteases in total that are linked to pancreatic cancer progression will allow us to obtain statistically significant measurements within 1 hour.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government