DEVELOPMENT OF A RAPID IN VITRO SCREENING ASSAY FOR DETECTION OF CANCER BIOMARKERS IN URINE AND BLOOD.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N43CO110069
Agency Tracking Number: N43CO110069
Amount: $200,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2011
Solicitation Year: 2011
Solicitation Topic Code: NCI
Solicitation Number: N/A
Small Business Information
1310 RESEARCH PK DR, MANHATTAN, KS, 66502-5000
DUNS: 946856804
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 FRANK KROH
 (785) 537-0179
 MADISONA@NANOSCALECORP.COM
Business Contact
 FRANK KROH
Phone: (785) 537-0179
Email: MADISONA@NANOSCALECORP.COM
Research Institution
N/A
Abstract
The goal of the project is to develop a rapid in vitro screening assay for detection of cancer biomarkers in urine and blood. The aim is testing and optimization of nanoparticle sensors, based on cyanine dyes that are chemically linked to Fe/Fe3O4 nanoparticles via protease-selective consensus (cleavage) sequences. The focus is on the quantitative determination of active cancer-specific proteases in urine and blood via simple fluorescence measurements. The severing from the nanoparticle stops the efficientfluorescence quenching of the organic luminophore by the nanoparticle, thus allowing the detection of the protease activity. The matrix metalloproteinases, urokinase-type plasminogen activator, and cathepsins, are up-regulated in the vast majority of progressing cancers and can, therefore, serve as markers for cell survival/tumor progression, angiogenesis, and tissue remodeling/ invasion. The focus will be on pancreatic cancer, and the studies will be done on urine and blood from 30 patients and 12 healthy individuals. Measuring twelve proteases in total that are linked to pancreatic cancer progression will allow us to obtain statistically significant measurements within 1 hour.

* Information listed above is at the time of submission. *

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