SBIR TOPIC 294 PHASE I - DEVELOPMENT OF GLYCOSYLATION-SPECIFIC RESEARCH REAGENTS (ANTIBODIES AND APTAMERS)

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$147,520.00
Award Year:
2011
Program:
SBIR
Phase:
Phase I
Contract:
N43CO110073
Agency Tracking Number:
N43CO110073
Solicitation Year:
2011
Solicitation Topic Code:
NCI
Solicitation Number:
n/a
Small Business Information
DETROIT RANDD, INC.
4683 RAVINE DR, BLOOMFIELD HILLS, MI, 48301-3640
Hubzone Owned:
N
Minority Owned:
Y
Woman Owned:
Y
Duns:
030673508
Principal Investigator:
HYESOOK KIM
(248) 539-3236
HSKIM@AOL.COM
Business Contact:
HYESOOK KIM
(248) 539-3236
HSKIM@AOL.COM
Research Institution:
n/a
Abstract
Protein O-GlcNAcylation (N-acetylglucosamine modification), which decreases phosphorylation, plays a critical role in cell-cycle regulation, apoptosis and signal transduction. So far, ~120 O-GlcNAcylated human proteins have been found. To elucidate functions of O-GlcNAcylation, it is necessary to develop site-specific antibodies for O-GlcNAcylated proteins. Many pan-specific antibodies for O-GlcNAcylation are available. However, few site-specific antibodies for O-GlcNAcylated proteins have been successfullyproduced. During the Phase I study, we will synthesize multiple antigen peptides with various features against O-GlcNAcylation sites of c-myc and p53 to test effects of a specific peptide design on the sensitivity and specificity of the antibodies. Sensitivity and specificity of the antibodies will be determined to find out the best peptide design. We will explore utility of the O-GlcNAcylation site-specific antibodies using human breast cell lines, normal (MCF10A) and tumor cells (MCF-7 and N-ras and H-ras MCF10A) with and without treatment with various O-GlcNAcase inhibitors.

* information listed above is at the time of submission.

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