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Microfocused Nanoparticulate ions to enable intracellular molecular imaging.

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44DA030853-02
Agency Tracking Number: R44DA030853
Amount: $2,387,081.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: NIDA
Solicitation Number: N/A
Solicitation Year: 2011
Award Year: 2011
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
3401 Louisiana St., Suite 355
United States
DUNS: 154074553
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (713) 522-9880
Business Contact
Phone: (713) 522-9880
Research Institution

DESCRIPTION (provided by applicant): Within the volume of a single cell, lines an extensive amount of information. The current scope of molecular imaging using mass spectrometry typically swamps the cell volume with organic acid matrix just to get observable signals. Herein lies the problem. Putting more matrix that there is analyte (i.e. cellular volume) inherently makes intracellular imaging via organic acids improbable. Therefore, Ionwerks has developed a comprehensive set of instrumentation, and nanoparticulate preparation techniques which combine two dimensional ion-mobility mass spectrometry and nanoparticulate tissue preparation which provide a means to analysis of tissues while preserving their histological attributes. Our research has demonstrated that sub-monolayers of nanoparticulates implanted into various depths within a tissue, provide agreeable signal levels. This means, the particulate to analyte ratio is much less that typical preparations: the chemical information is preserved and observable. The nanoparticulates will also be microfocused to allow for unique molecular secondary ion mass spectrometry (SIMS). Furthermore, we are developing small format oTOF MS detectors which can be combined with leading edge surface imaging techniques to create a one of a kind imaging platform from which surfaces / tissues can be morphologically imaged, and mass spectra of ejected ions can be recorded. PUBLIC HEALTH RELEVANCE: The goal of this project is implant histological prepared tissues with gold nanoparticulates, then to measure the ejected ions using a low profile oTOF-MS system from a microfocused nanoparticulate beam. This project will lay the groundwork for utilizing the helium ion-beam microprobe systems as a combined topographical imaging system, as well as molecular imaging systems.

* Information listed above is at the time of submission. *

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