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High-capacity and Cost-effective Manufacture of Chloroperoxidase

Award Information
Agency: Department of Defense
Branch: Army
Contract: W911NF-11-C-0238
Agency Tracking Number: A11A-014-0173
Amount: $95,754.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: A11a-T014
Solicitation Number: 2011.A
Timeline
Solicitation Year: 2011
Award Year: 2011
Award Start Date (Proposal Award Date): 2011-09-29
Award End Date (Contract End Date): N/A
Small Business Information
9 Tabor Hill Rd, Lincoln, MA, 01773-
DUNS: 084592042
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 David Easson
 Director, BioProcess Division
 (508) 344-9719
 deasson@impact-td.com
Business Contact
 William Cox
Title: Vice President
Phone: (508) 951-2436
Email: bcox@impact-td.com
Research Institution
 University of Massachusetts Lowell
 Carl Lawton
 One University Avenue
Lowell, MA, 01854-
 (978) 944-0766
 Nonprofit college or university
Abstract
Bioengineering offers an efficient and robust route for the production of enzymatic catalysts such as chloroperoxidase (CPO). The CPO from Caldariomyces fumago can catalyze non-specific halogenation of electrophilic organic molecules, epoxidation and hydroxylation of olefins and organic sulfides, and generation of free chlorine from chloride ion. The objectives are to: A. Develop a Caldariomyces fumago expression system and purification scheme to produce milligram quantities of CPO at a purity of 90% or greater. Specifcally, we will carry out Experimental Design techniques to develop a more balanced approach to fermentation improvement. We will use screening designs to evaluate the important factors and interactions on the concentration of CPO, rate of production and purity. The important factors can then be evaluated in a statistically designed optimization study. We will also develop a low cost purification scheme to produce CPO at a purity greater than 90% B. Perform detailed biochemical characterization of the purified enzyme (CPO). We will determine the enzyme's catalytic efficiency and stability under reaction conditions suitable for field use. This will entail identification of model substrates and standardized assay conditions. We will also determine the storage stability (shelf life) of CPO preparations under various environmental conditions.

* Information listed above is at the time of submission. *

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