In Vivo Expansion of Human Hepatocytes in FRG Mice

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 2R42RR028190-02
Agency Tracking Number: R42RR028190
Amount: $773,666.00
Phase: Phase II
Program: STTR
Awards Year: 2011
Solitcitation Year: 2011
Solitcitation Topic Code: NCRR
Solitcitation Number: N/A
Small Business Information
YECURIS CORPORATION
3181 SW SAM JACKSON PARK RD, L321, PORTLAND, OR, 97239-3098
Duns: 801452249
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 MARKUS GROMPE
 (503) 494-6888
 grompem@ohsu.edu
Business Contact
 JOHN BIAL
Phone: (503) 494-9597
Email: johnbial@yecuris.com
Research Institution
 OREGON HEALTH AND SCIENCE UNIVERSITY
 Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): The FDA requires the use of human hepatocytes for preclinical drug testing by pharmaceutical researchers, but unfortunately the supply of these cells is severely limited. Currently, cadaveric donors and surgical liver resection specimens are currently the only available source of human hepatocytes. These samples are very scarce and often yield cells of poor quality unsuitable for research. We have developed technology to massively expand fully functional primary human hepatocytes by serial transplantation in immune deficient FRGN mice in vivo. Hepatocytes isolated from these animals are highly viable and functional. Cell farming in FRGN mice allows the production of an unlimited supply of human hepatocytes for research in toxicology, drug metabolism, infectious disease, and cancer biology. The aims of this application are to optimize the isolation and storage of farmed hepatocytes and to fully validate their utility in comparison to the currently available cells. Once fully validated, these cells are going to be valuable in the commercial market. PUBLIC HEALTH RELEVANCE: The successful implementation of human hepatocyte farming technology will significantly impact human health by providing this vital resource for drug development at a higher quality and lower cost than is currently available.

* information listed above is at the time of submission.

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