TAS::75 0849::TAS OPTION EXERCISE MODIFICATION

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$373,298.00
Award Year:
2011
Program:
SBIR
Phase:
Phase II
Contract:
N44CO090067
Award Id:
n/a
Agency Tracking Number:
N44CO090067
Solicitation Year:
2011
Solicitation Topic Code:
NCI
Solicitation Number:
n/a
Small Business Information
2155 E CONFERENCE DR STE 104, TEMPE, AZ, 85284-2604
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
965127038
Principal Investigator:
KEMMONS TUBBS
(480) 804-1778
KTUBBS@INTRINSICBIO.COM
Business Contact:
KEMMONS TUBBS
(480) 804-1778
KTUBBS@INTRINSICBIO.COM
Research Institution:
Stub




Abstract
This proposal sets forth a precise and rapid methodology for identifying the interaction sites between monoclonal antibodies and proteins. The basis of the system is a high-throughput mass spectrometry-based analysis of epitope determinants to identify theinteracting protein sequences using immunoaffinity capture combined with enzymatic digestion of the target protein; either pre- or post-capture. This combination will be used to rapidly determine the approximate location of each binding epitope. The precise location of the binding epitope will then be determined by immunoaffinity capture and mass spectrometric readout of sets of synthetic ladder peptides that span the approximate epitope established from the initial analysis. The project will develop reproducible methods to demonstrate epitope mapping for monoclonal antibodies of interest to NCI tested by multiple sampling and quantifiable results. Mapped epitopes will be functionally characterized by amino acid substitutions in the epitope sequence using high-throughput spotted array surface plasmon resonance imaging and SPR-Biomolecular Interaction Analysis to identify the key amino acids that are critically involved in binding the antibody to the antigenic epitope. Thus, this approach will provide an efficient and cost effective platform from which to accomplish high-throughput epitope mapping of monoclonal antibodies for use in diagnosis of cancer.

* information listed above is at the time of submission.

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