Spores Viability Detection System

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: W911NF-11-C-0095
Agency Tracking Number: C111-101-0025
Amount: $99,996.00
Phase: Phase I
Program: SBIR
Awards Year: 2011
Solicitation Year: 2011
Solicitation Topic Code: CBD11-101
Solicitation Number: 2011.1
Small Business Information
Physical Optics Corporation
Photonic Systems Division, 20600 Gramercy Place, Bldg. 100, Torrance, CA, -
DUNS: 153865951
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 Gregory Zeltser
 Principal Scientist
 (310) 320-3088
 psproposals@poc.com
Business Contact
 Gordon Drew
Title: Chief Financial Officer
Phone: (310) 320-3088
Email: gedrew@poc.com
Research Institution
N/A
Abstract
There is an urgent need for a rapid biological detection system that demonstrates the efficacy of decontamination products for inactivating Bacillus anthracis spores on environmental surfaces. To address the U.S. Army/CBD need for such a fieldable, rapid, NIST-certified, quantitative bio-indicator system, Physical Optics Corporation (POC) proposes to develop a new Spores Viability Detection (SVD) system that consists of (1) spore strips made of different materials that are inoculated with Bacillus thuringiensis Al Hakam spores and (2) a hand-held fluorometer. The SVD system will be a fieldable, rapid (35 min), sensitive, reproducible tool for assessment of the viability of decontaminated indicator spores in an easy-to-perform three-step assay protocol. In Phase I, POC will demonstrate the feasibility of the SVD system by developing a reproducible spore preparation protocol that meets the Army/CBD requirements, fabricating a fieldable fluorometer prototype, and demonstrating its ability to quantitatively measure live/dead spores after viability staining. In Phase II, POC plans to optimize the SVD system design and performance parameters including sensitivity and accuracy of the fluorometer and development of spore strips made of different materials that are inoculated with 6, 7, and 8 logs of the spores.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government