Development of an in vitro assay as correlate of passive immune protection against botulinum neurotoxin to minimize use of whole animal testing

Award Information
Agency:
Department of Defense
Branch
Office for Chemical and Biological Defense
Amount:
$149,949.00
Award Year:
2011
Program:
SBIR
Phase:
Phase I
Contract:
W81XWH-11-C-0085
Award Id:
n/a
Agency Tracking Number:
C111-109-0018
Solicitation Year:
2011
Solicitation Topic Code:
CBD11-109
Solicitation Number:
2011.1
Small Business Information
5754 Pacific Center Blvd Suite 204, San Diego, CA, -
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
129852864
Principal Investigator:
Eric Ervin
Vice President R&D - EBS Utah
(801) 582-0146
eervin@electronicbio.com
Business Contact:
Tracey Wrightson
Director of Operations
(858) 412-1800
twrightson@electronicbio.com
Research Institution:
Stub




Abstract
This Small Business Innovation Research Phase I project will develop a non-animal based in-vitro assay for determining the efficacy of Botulinum (BoNT) neutralizing therapeutics; the dual nanopore assay (DNPA). New technologies are currently needed in order to facilitate the development and approval of BoNT vaccines and therapies, and align with the NIH and FDA"s guidance for reducing/replacing the number of vertebrate animals used in medical testing. Currently, the mouse lethality neutralization (MLN) assay is the standard test for determining the efficacy of BoNT neutralizing therapeutics. However, the inherent variability in sensitivity of the MLN assay due to different mouse species, test conditions, and the need to utilize live animal testing severely limit its accuracy and utility. EBS"s DNPA will be capable of monitoring the complete mechanism of all seven BoNT isoforms, making rapid analysis of BoNT neutralizing therapeutics, including complex mixtures, possible.

* information listed above is at the time of submission.

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