BioPrep: A Novel Microfluidic Cartridge for Universal Bio-sample Preparation

Award Information
Agency: Department of Defense
Branch: Army
Contract: W911NF-11-C-0063
Agency Tracking Number: A102-074-0703
Amount: $69,998.00
Phase: Phase I
Program: SBIR
Awards Year: 2011
Solicitation Year: 2010
Solicitation Topic Code: A10-074
Solicitation Number: 2010.2
Small Business Information
215 Wynn Dr., 5th Floor, Huntsville, AL, -
DUNS: 185169620
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 Ketan Bhatt
 Senior Research Engineer
 (256) 327-0666
Business Contact
 Deborah Phipps
Title: Contracts Manager
Phone: (256) 726-4884
Research Institution
A critical but often overlooked aspect of bioagent detection is rendering the complex environmental sample ready for detection. Commercially available sample preparation technologies are time-consuming, high cost and require trained personnel and sophisticated equipment for operation and thus are not feasible for field use. Furthermore, sample preparation technologies need to accommodate a variety of input samples (e.g., water, soil, blood) and a host of detection moieties (e.g., DNA, RNA, proteins, peptides, whole cells). Ideally, a fieldable bio-sample preparation platform should be rapid, compact, reagent-less, automated, require minimal user training, and universal. We propose to develop a general purpose BioPrep cartridge for universal bio-sample preparation for detection of biowarfare agents. The cartridge will be based on CFDRC"s EMUS platform a suite of microfluidic component technologies for electrokinetics-based sample preparation. It features modular design, offering a high degree of customization and electric field driven, unified physics for ease of integration and enhanced fieldability. Proof-of-concept during Phase I will be demonstrated by separating individual classes (e.g., spores, vegetative bacteria, viruses, DNA, proteins) of bioagents from heterogeneous samples. During Phase II, the individual component technologies will be optimized and integrated. The integrated microfluidic cartridge will be demonstrated for a variety of complex environmental samples.

* Information listed above is at the time of submission. *

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