Manufacturing Development of Allogeneic Stem Cells in Clinical Settings
Small Business Information
7200 Highway 150, Greenville, IN, -
AbstractTechshot proposes to improve the isolation and immunoselection of mesenchymal stem cells from adipose tissue using Quadrupole Magnetic flow Sorting (QMS). This separator technology sorts magnetically labeled cells at high speed and is capable of processing large volumes of tissue such as lipoaspirate digests. QMS has previously been employed to isolate hematological cells from blood and Pancreatic Islets of Langerhans from digested solid tissue, and the innovative new embodiment will be capable of isolating single cells from solid tissue. The ability to produce large numbers of pure cells by QMS could minimize in vitro expansion requirements and automate the cell production process. In proposed Phase I research Techshot will pursue the following objectives: (1) Design and prototype a closed system to isolate and enrich with high specificity donor stem cells from adipose tissue; (2) Satisfy requirements for labeling and selecting one donor stem cell type; and (3) Determine the flexibility to employ QMS for multiple applications by testing with adipose tissue. This unique immunological based approach is expected to result in leap-ahead improvements in the efficiency of enrichment, quality, and specificity for allogeneic cell therapies utilizing adipose mesenchymal cells as an exemplary source for post-trauma tissue regeneration and wound healing.
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