Development of a Molecular Assay to Identify Ticks that Vector Rickettsial Diseases

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-11-C-0040
Agency Tracking Number: A103-154-0106
Amount: $69,902.00
Phase: Phase I
Program: SBIR
Awards Year: 2011
Solitcitation Year: 2010
Solitcitation Topic Code: A10-154
Solitcitation Number: 2010.3
Small Business Information
Streamline Automation, LLC
3100 Fresh Way SW, Huntsville, AL, -
Duns: 124289294
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Douglas Prasher
 Senior Scientist
 (256) 713-1220
 Douglas.Prasher@StreamlineAutomatio
Business Contact
 Alton Reich
Title: Lead Engineer
Phone: (256) 713-1220
Email: Alton.Reich@StreamlineAutomation.bi
Research Institution
 Stub
Abstract
Assignment of a taxonomic species to a tick specimen pulled from a person is a non-trivial exercise even for a trained taxonomist let alone for a typical health care provider. The nearly 700 species in the taxonomic family Ixodidae, sometimes referred to as hard-backed ticks, are those of most concern to health care professionals. We propose here to develop an innovative molecularly-based methodology that should be able to be performed by personnel with minimal laboratory training (i.e. college laboratory course in either biochemistry, molecular biology, or microbiology). It involves the use of real-time PCR on commercially-available equipment and will be capable of identifying 100's of species. Further automation of the process during Phase 2 will make the detection equipment operable by personnel in the field with a few hours of targeted, specific training.

* information listed above is at the time of submission.

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