A High-Throughput Blood Esterase Panel Assay

Award Information
Agency:
Department of Defense
Branch
Office for Chemical and Biological Defense
Amount:
$750,000.00
Award Year:
2011
Program:
SBIR
Phase:
Phase II
Contract:
W81XWH-10-C-0063
Agency Tracking Number:
C2-0348
Solicitation Year:
2010
Solicitation Topic Code:
CBD10-108
Solicitation Number:
2010.1
Small Business Information
Agave BioSystems, Inc.
P.O. Box 100, Ithaca, NY, -
Hubzone Owned:
Y
Socially and Economically Disadvantaged:
Y
Woman Owned:
N
Duns:
022552900
Principal Investigator:
Julien Fey
Senior Scientist
(607) 272-0002
jfey@agavebio.com
Business Contact:
Noe Salazar
President
(512) 373-8601
nsalazar@agavebio.com
Research Institution:
Stub




Abstract
Organophosphorus chemical warfare nerve agents (OP-CWA) are attractive to terrorist groups and rogue states as an inexpensive and accessible technology for chemical warfare. OP-CWA and organophosphate pesticides cause severe neurological symptoms and death by inhibiting the enzyme acetylcholinesterase (AChE); the resulting excess acetylcholine accumulates and overstimulates the human or animal body. Other esterases and organophosphatases in the blood either bind (butyrylcholinesterase, BChE) or catalytically degrade (paraoxonase, PON1) OP-CWA. Therefore, it is critically important to be able to quickly monitor the human blood complement of OP-CWA enzymes to assess both susceptibility and current condition. For military and civilian clinical use, a high-throughput, minimally invasive assay system is needed to quickly and accurately screen large numbers of soldiers/agricultural workers/first responders for OP-CWA or OP-pesticide exposure. The currently available WRAIR assay is high-throughput, uses a minimal amount of whole blood, and is sensitive and accurate for AChE and BChE levels. An assay that additionally measures PON1 and albumin esterase activities relevant to OP degradation and/or binding is greatly needed to more fully assess OP-CWA or -pesticide susceptibility for individuals at risk.

* information listed above is at the time of submission.

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