Handheld Aptamer-Magnetic Bead-Quantum Dot Sensor for Leishmania

Award Information
Agency:
Department of Defense
Branch
Defense Health Program
Amount:
$709,977.00
Award Year:
2011
Program:
SBIR
Phase:
Phase II
Contract:
W81XWH-10-C-0179
Award Id:
n/a
Agency Tracking Number:
O2-1098
Solicitation Year:
2009
Solicitation Topic Code:
OSD09-H21
Solicitation Number:
2009.3
Small Business Information
4100 N. W. Loop 410, Suite 230, San Antonio, TX, 78229-4253
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
154088710
Principal Investigator:
JohnBruno
Senior Scientist
(210) 731-0015
john.bruno@otcorp.com
Business Contact:
WilliamHenderson
Chief Financial Officer
(210) 731-0015
bhenders@otcorp.com
Research Institute:
Stub




Abstract
Operational Technologies Corporation (OpTech) has completed a successful Phase I SBIR contract in which 72 new candidate DNA aptamer sequences against Leishmania major were developed and screened by ELISA-like plate assay. Several pilot assays were developed from the highest affinity candidate aptamers using a commercially available handheld and battery-operated fluorometer. In Phase II, OpTech plans to fully develop and deliver to the DoD two or more rapid (15 minute) ultrasensitive one-step plastic-adherent DNA aptamer-magnetic bead (MB) fluorescent sandwich assays (Bruno et al., J. Fluorescence 19:427-435, 2009) for L. major and L. infantum and a visceral leishmaniasis (39 kD kinesin) aptamer assay. In Phase I, this assay format demonstrated detection of Campylobacter and Listeria to<10 cfu/mL and between 10-100 Leishmania promastigotes. OpTech will optimize its lyophilized assay performance with respect to specificity, sensitivity and shelf life of>2 years at 35oC by systematic study of DNA aptamer, magnetic bead, and fluorescent nanoparticle reagent concentrations. OpTech will also evaluate trehalose or other excipient level effects along with innovative vacuum or air-tight packaging of the lyophilized assays. Finally, OpTech will test the assays against various species of live Leishmania and compare to gold standard ELISA and PCR assays.

* information listed above is at the time of submission.

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