A Point-of-Care Field Assay for Dengue Viruses

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$780,000.00
Award Year:
2011
Program:
SBIR
Phase:
Phase II
Contract:
W81XWH-10-C-0038
Award Id:
n/a
Agency Tracking Number:
A2-4447
Solicitation Year:
2009
Solicitation Topic Code:
A09-155
Solicitation Number:
2009.3
Small Business Information
P.O. Box 100, Ithaca, NY, -
Hubzone Owned:
Y
Minority Owned:
N
Woman Owned:
N
Duns:
022552900
Principal Investigator:
Joel Tabb
Principal Scientist
(607) 272-0002
jtabb@agavebio.com
Business Contact:
Noe Salazar
President
(512) 656-6200
nsalazar@agavebio.com
Research Institution:
Stub




Abstract
Dengue viruses are transmitted by mosquito vectors throughout tropical and subtropical regions of Asia, Africa, and Central and South America. Four serotypes circulate, DEN-1 through -4, and immunity to one type does not preclude illness from another type and can exacerbate subsequent disease with another type. While dengue fever can be a mild infection, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) cause death at a high rate and are associated with multiple serotypes worldwide. Dengue fever symptoms, especially at the early acute stage, can resemble infection with other disease agents, such as malaria and rickettsial diseases, with different treatment regimens. Loop-mediated isothermal amplification (LAMP) is a recently developed technique with similar or better detection sensitivity and specificity compared to PCR assays, but allows a single temperature incubation and simple readout more suitable to field devices. LAMP also offers the potential of detection in less pure samples, such as blood, facilitating quick field sample preparation. Therefore, Agave BioSystems proposes to develop a real-time reverse-transcriptase loop-mediated isothermal amplification (real-time RT-LAMP) diagnostic for detection of Dengue viruses in human serum.

* information listed above is at the time of submission.

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